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    DNASTAR megalign computer program
    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR <t>MegAlign</t> computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.
    Megalign Computer Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 10381 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megalign computer program/product/DNASTAR
    Average 99 stars, based on 10381 article reviews
    megalign computer program - by Bioz Stars, 2026-04
    99/100 stars

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    1) Product Images from "Evolution and function of calponin and transgelin."

    Article Title: Evolution and function of calponin and transgelin.

    Journal: Frontiers in cell and developmental biology

    doi: 10.3389/fcell.2023.1206147

    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.
    Figure Legend Snippet: FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.

    Techniques Used: Generated, Software, Sequencing, Derivative Assay, Western Blot

    FIGURE 2 Phylogenetic tree of vertebrate transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. TAGLN, TAGLN2, and TAGLN3 isoforms are marked in purple, red and green, respectively. The NCBI database accession numbers of the sequences analyzed are: African clawed frog TAGLN, NP_001083600.1; African clawed frog TAGLN2 NP_001080783.1; African turquoise killifish TAGLN3, KAF7213900.1; Black-legged kittiwake TAGLN, XP_054078918.1; Black-legged kittiwake TAGLN2, XP_054038817.1; Black-legged kittiwake TAGLN3, XP_054071416.1; Cape cliff lizard TAGLN, XP_053124973.1; Cape cliff lizard TAGLN2, XP_053133737.1; Cape cliff lizard TAGLN3, XP_053167629.1; Chicken TAGLN, AAA48782.1; Chicken TAGLN2, XP_024999416.1; Chicken TAGLN3, XP_040518015.1; Diamondback rattlesnake TAGLN, JAI10881.1; European plaice TAGLN, XP_053298355.1; European seabass TAGLN, XP_051257332.1; Green swordtail TAGLN3, KAF5894368.1; Human TAGLN, NP_001001522.1; Human TAGLN2, KAI4083433.1; Human TAGLN3, KAI2530827.1; Mexican tetra TAGLN2, XP_049332440.1; Mexican tetra TAGLN3, KAG9283353.1; Mouse TAGLN, CAA92941.1; Mouse TAGLN2, NP_848713.1; Mouse TAGLN3, AAH55338.1; Orange spotted grouper TAGLN, ABW04145.1; Tiger rattlesnake TAGLN, 2, XP_039222054.1; Tiger rattlesnake TAGLN3, XP_039220015.1; Tiny caecilian TAGLN, XP_030076731.1; Tiny caecilian TAGLN2, XP_030043052.1; Tiny caecilian TAGLN3, XP_030060064.1; Two-lined caecilian TAGLN, XP_029429188.1; Two-lined caecilian TAGLN2, XP_XP_ 029436331.1; Two-lined caecilian TAGLN3, XP_029434652.1; Western clawed frog TAGLN, NP_001025579.1; Western clawed frog TAGLN2, NP_ 989354.1; Yellow drum fish TAGLN, KAG8008337.1.
    Figure Legend Snippet: FIGURE 2 Phylogenetic tree of vertebrate transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. TAGLN, TAGLN2, and TAGLN3 isoforms are marked in purple, red and green, respectively. The NCBI database accession numbers of the sequences analyzed are: African clawed frog TAGLN, NP_001083600.1; African clawed frog TAGLN2 NP_001080783.1; African turquoise killifish TAGLN3, KAF7213900.1; Black-legged kittiwake TAGLN, XP_054078918.1; Black-legged kittiwake TAGLN2, XP_054038817.1; Black-legged kittiwake TAGLN3, XP_054071416.1; Cape cliff lizard TAGLN, XP_053124973.1; Cape cliff lizard TAGLN2, XP_053133737.1; Cape cliff lizard TAGLN3, XP_053167629.1; Chicken TAGLN, AAA48782.1; Chicken TAGLN2, XP_024999416.1; Chicken TAGLN3, XP_040518015.1; Diamondback rattlesnake TAGLN, JAI10881.1; European plaice TAGLN, XP_053298355.1; European seabass TAGLN, XP_051257332.1; Green swordtail TAGLN3, KAF5894368.1; Human TAGLN, NP_001001522.1; Human TAGLN2, KAI4083433.1; Human TAGLN3, KAI2530827.1; Mexican tetra TAGLN2, XP_049332440.1; Mexican tetra TAGLN3, KAG9283353.1; Mouse TAGLN, CAA92941.1; Mouse TAGLN2, NP_848713.1; Mouse TAGLN3, AAH55338.1; Orange spotted grouper TAGLN, ABW04145.1; Tiger rattlesnake TAGLN, 2, XP_039222054.1; Tiger rattlesnake TAGLN3, XP_039220015.1; Tiny caecilian TAGLN, XP_030076731.1; Tiny caecilian TAGLN2, XP_030043052.1; Tiny caecilian TAGLN3, XP_030060064.1; Two-lined caecilian TAGLN, XP_029429188.1; Two-lined caecilian TAGLN2, XP_XP_ 029436331.1; Two-lined caecilian TAGLN3, XP_029434652.1; Western clawed frog TAGLN, NP_001025579.1; Western clawed frog TAGLN2, NP_ 989354.1; Yellow drum fish TAGLN, KAG8008337.1.

    Techniques Used: Generated, Western Blot

    FIGURE 3 Phylogenetic tree of invertebrate calponin isoforms. The phylogenetic tree of invertebrate calponin was generated by aligning the amino acid sequences of annotated calponin isoforms in the representative invertebrate species with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. Calponin isoforms 1, 2, and 3 are indicated in purple, red and green fonts, respectively. The NCBI database accession numbers of the sequences analyzed are: Bark spider CNN2, GIY95920.1; Bat sea star CNN1, XP_038068192.1; Bat sea star CNN3, XP_038068193.1; Belted springtail CNN1, ODN05277.1; Bird tapeworm CNN3, JAP59453.1; Blood fluke CNN3, XP_012795279.2; Dog round worm CNN2, KHN73181.1; Dog tapeworm CNN1, KAH9282893.1; Dog tapeworm CNN3, EUB56891.1; East Asian octopus CNN1, XP_029648512.1; Fungivorous round worm CNN2, KAH7720906.1; Garden spider CNN3, GBN55272.1; Gray mussel CNN2, APB61452.1; Hood coral CNN1, PFX34187.1; Hood coral CNN3, PFX34186.1; Human whip worm CNN1, CDW60276.1; Intoshia linei worm CNN1, OAF71457.1; Japanese scallop CNN3, OWF41392.1; Oriental liver fluke CNN3, GAA43144.2; Pale anemone CNN1,KXJ22199.1; Taxon placozoa CNN1, RDD43626.1; Water flea CNN3, JAM94970.1; White springtail CNN1, OXA37663.1; Satsuma tube worm CNN3, KAI0207068.1; Salmon louse CNN3, ACO12641.1; Sea louse CNN3, ACO14747.1; Sea spider CNN3, KAG1690965.1; Spider mite CNN3, XP_015791637.1.
    Figure Legend Snippet: FIGURE 3 Phylogenetic tree of invertebrate calponin isoforms. The phylogenetic tree of invertebrate calponin was generated by aligning the amino acid sequences of annotated calponin isoforms in the representative invertebrate species with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. Calponin isoforms 1, 2, and 3 are indicated in purple, red and green fonts, respectively. The NCBI database accession numbers of the sequences analyzed are: Bark spider CNN2, GIY95920.1; Bat sea star CNN1, XP_038068192.1; Bat sea star CNN3, XP_038068193.1; Belted springtail CNN1, ODN05277.1; Bird tapeworm CNN3, JAP59453.1; Blood fluke CNN3, XP_012795279.2; Dog round worm CNN2, KHN73181.1; Dog tapeworm CNN1, KAH9282893.1; Dog tapeworm CNN3, EUB56891.1; East Asian octopus CNN1, XP_029648512.1; Fungivorous round worm CNN2, KAH7720906.1; Garden spider CNN3, GBN55272.1; Gray mussel CNN2, APB61452.1; Hood coral CNN1, PFX34187.1; Hood coral CNN3, PFX34186.1; Human whip worm CNN1, CDW60276.1; Intoshia linei worm CNN1, OAF71457.1; Japanese scallop CNN3, OWF41392.1; Oriental liver fluke CNN3, GAA43144.2; Pale anemone CNN1,KXJ22199.1; Taxon placozoa CNN1, RDD43626.1; Water flea CNN3, JAM94970.1; White springtail CNN1, OXA37663.1; Satsuma tube worm CNN3, KAI0207068.1; Salmon louse CNN3, ACO12641.1; Sea louse CNN3, ACO14747.1; Sea spider CNN3, KAG1690965.1; Spider mite CNN3, XP_015791637.1.

    Techniques Used: Generated

    FIGURE 4 Phylogenetic tree of invertebrate animal and fungal transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of transgelin isoforms of representative invertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are listed in purple, red and green fonts, respectively. Fungal transgelins are highlighted in brown font. The NCBI database accession numbers of the sequences are: California two spot octopus TAGLN3, XP_014777580.1; Caenorhabditis elegans TAGLN, NP_ 493713.2; Common house spider TAGLN, LAA01862.1; Common starfish TAGLN, QAA95982.1; Darwini’s bark spider TAGLN3, GIY80101.1; Dog tapeworm TAGLN, KAH9282095.1; Oriental liver fluke TAGLN, KAG5444608.1; Freshwater snail TAGLN3, KAH9519225.1; Hood coral TAGLN2, PFX34185.1; Human scabies TAGLN2, KAI7688587.1; Japanese scallop TAGLN3, OWF50751.1; Mediterranean mussel TAGLN, VDI10566.1; Oriental lung fluke TAGLN, KAA3679206.1; Pharaoh cuttlefish TAGLN, CAE1295289.1; Pork worm TAGLN2, KRY33709.1; Satsuma tube worm TAGLN2, KAI0231824.1; Sea slug TAGLN, GFO13790.1; Silkworm moth TAGLN, ABF51271.1; Speckled wood butterfly TAGLN, JAA92459.1; Strawberry crimp disease nematode TAGLN, KAI6193121.1; Water flea TAGLN3, JAJ10604.1; Aspergillus luchuensis TAGLN, GAT26957.1; Budding yeast TAGLN, KAF4004496.1; Candida parapsilosis TAGLN, KAI5906036.1; Fission yeast TAGLN, O14185.1.
    Figure Legend Snippet: FIGURE 4 Phylogenetic tree of invertebrate animal and fungal transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of transgelin isoforms of representative invertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are listed in purple, red and green fonts, respectively. Fungal transgelins are highlighted in brown font. The NCBI database accession numbers of the sequences are: California two spot octopus TAGLN3, XP_014777580.1; Caenorhabditis elegans TAGLN, NP_ 493713.2; Common house spider TAGLN, LAA01862.1; Common starfish TAGLN, QAA95982.1; Darwini’s bark spider TAGLN3, GIY80101.1; Dog tapeworm TAGLN, KAH9282095.1; Oriental liver fluke TAGLN, KAG5444608.1; Freshwater snail TAGLN3, KAH9519225.1; Hood coral TAGLN2, PFX34185.1; Human scabies TAGLN2, KAI7688587.1; Japanese scallop TAGLN3, OWF50751.1; Mediterranean mussel TAGLN, VDI10566.1; Oriental lung fluke TAGLN, KAA3679206.1; Pharaoh cuttlefish TAGLN, CAE1295289.1; Pork worm TAGLN2, KRY33709.1; Satsuma tube worm TAGLN2, KAI0231824.1; Sea slug TAGLN, GFO13790.1; Silkworm moth TAGLN, ABF51271.1; Speckled wood butterfly TAGLN, JAA92459.1; Strawberry crimp disease nematode TAGLN, KAI6193121.1; Water flea TAGLN3, JAJ10604.1; Aspergillus luchuensis TAGLN, GAT26957.1; Budding yeast TAGLN, KAF4004496.1; Candida parapsilosis TAGLN, KAI5906036.1; Fission yeast TAGLN, O14185.1.

    Techniques Used: Generated, Software

    FIGURE 8 Phylogeny of the calponin-transgelin superfamily genes in vertebrates. The combined phylogenetic tree of vertebrate calponin and transgelin isoforms was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are marked in purple, red and green fonts, respectively. The vertebrate calponin isoforms 1, 2, and 3 are clustered in the blue box. The phylogenetic lineages demonstrate that calponin and transgelin diverged early during vertebrate evolution and transgelin-1 emerged before transgelin-2, transgelin-3 and the three calponin isoforms. The NCBI database accession numbers of the sequences are listed in the legends of Figures 1, 2.
    Figure Legend Snippet: FIGURE 8 Phylogeny of the calponin-transgelin superfamily genes in vertebrates. The combined phylogenetic tree of vertebrate calponin and transgelin isoforms was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are marked in purple, red and green fonts, respectively. The vertebrate calponin isoforms 1, 2, and 3 are clustered in the blue box. The phylogenetic lineages demonstrate that calponin and transgelin diverged early during vertebrate evolution and transgelin-1 emerged before transgelin-2, transgelin-3 and the three calponin isoforms. The NCBI database accession numbers of the sequences are listed in the legends of Figures 1, 2.

    Techniques Used: Generated, Software

    FIGURE 9 Overall phylogeny of calponin-transgelin genes in vertebrate and invertebrate animals. The combined phylogenetic tree of vertebrate and invertebrate calponin and transgelin was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Vertebrate calponin isoforms are in blue font, invertebrate calponins are in black font, vertebrate transgelins are in green font and invertebrate transgelins are in red font. The NCBI database accession numbers of the sequences are in the legends of Figures 1–4. The evolutionary lineages show that while calponin and transgelin have evolved into distinct and conserved isoforms in vertebrates, the invertebrate homologs are significantly less differentiated.
    Figure Legend Snippet: FIGURE 9 Overall phylogeny of calponin-transgelin genes in vertebrate and invertebrate animals. The combined phylogenetic tree of vertebrate and invertebrate calponin and transgelin was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Vertebrate calponin isoforms are in blue font, invertebrate calponins are in black font, vertebrate transgelins are in green font and invertebrate transgelins are in red font. The NCBI database accession numbers of the sequences are in the legends of Figures 1–4. The evolutionary lineages show that while calponin and transgelin have evolved into distinct and conserved isoforms in vertebrates, the invertebrate homologs are significantly less differentiated.

    Techniques Used: Generated, Software



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    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR <t>MegAlign</t> computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.
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    90
    DNASTAR megalign® program of the lasergene® bioinformatics computing suite
    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR <t>MegAlign</t> computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.
    Megalign® Program Of The Lasergene® Bioinformatics Computing Suite, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 90 stars, based on 1 article reviews
    megalign® program of the lasergene® bioinformatics computing suite - by Bioz Stars, 2026-04
    90/100 stars
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    computer program megalign  (DNASTAR)
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    DNASTAR computer program megalign
    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR <t>MegAlign</t> computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.
    Computer Program Megalign, supplied by DNASTAR, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/computer program megalign/product/DNASTAR
    Average 99 stars, based on 1 article reviews
    computer program megalign - by Bioz Stars, 2026-04
    99/100 stars
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    megalign® computer program  (DNASTAR)
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    DNASTAR megalign® computer program
    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR <t>MegAlign</t> computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.
    Megalign® Computer Program, supplied by DNASTAR, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/megalign® computer program/product/DNASTAR
    Average 90 stars, based on 1 article reviews
    megalign® computer program - by Bioz Stars, 2026-04
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    Image Search Results


    FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.

    Journal: Frontiers in cell and developmental biology

    Article Title: Evolution and function of calponin and transgelin.

    doi: 10.3389/fcell.2023.1206147

    Figure Lengend Snippet: FIGURE 1 Phylogenetic tree of vertebrate calponin and isoforms. The phylogenetic tree was generated by alignment of amino acid sequences of calponin isoforms in representative vertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using the Clustal W method. The sequence similarity-derived evolutionary lineages demonstrate that each of the calponin isoforms is conserved in the vertebrate phylum while the three isoforms have significantly diverged during vertebrate evolution. This pattern indicates the adaptation of the calponin isoforms to different cell functions and tissue environments. Calponin isoforms 1, 2, and 3 are marked in purple, red and green, respectively. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. The NCBI database accession numbers of the sequences analyzed are: African clawed frog CNN1, NP_001085014.1; African clawed frog CNN2, ABG49504.1; African clawed frog CNN3, NP_001080482.1; Atlantic salmon CNN1, NP_001139857.1; Atlantic salmon CNN2, NP_001133873.1; Atlantic salmon CNN3, NP_001133337.1; Band tailed pigeon CNN2, OPJ86593.1; Bighead croaker CNN1, TKS92442.1; Bighead croaker CNN2, TKS80042.1; Bighead croaker CNN3, TKS88103.1; Brown tree snake CNN2, JAG68493.1; Chimpanzee CNN1,NP_ 001267033.1; Chimpanzee CNN2, JAA13388.1; Chimpanzee CNN3, JAA44470.1; Chinese hamster CNN1, EGV96164.1; Chinese hamster CNN2, EGV99480.1; Chinese hamster CNN 3, EGW11625.1; Chicken CNN1, NP_990847.1; Chicken CNN2, NP_001135728.1; Chicken CNN3, NP_001341600.1; Copperhead snake CNN2, JAV51035.1; Eastern Diamondback rattlesnake CNN2, AFJ49586.1; Human CNN1, NP_001290.2; Human CNN2, AAI48265.1; Human CNN3, AAB35752.1; Mainland tiger snake CNN1, XP_026535408.1; Mouse CNN1, AAI38864.1; Mouse CNN2, EDL31614.1; Mouse CNN3, AAH85268.1; Naked mole CNN1, JAN96391.1; Naked mole CNN2, EHB16944.1; Naked mole CNN3, XP_004841280.1; Snakehead fish CNN1, KAF3700611.1; Snakehead fish CNN2, KAF3699176.1; Snakehead fish CNN3, KAF3703402.1; Western terrestrial garter snake CNN1, XP_032066879.1; Western clawed frog CNN1, NP_001015796.1; Western clawed frog CNN2, NP_998841.1; Western clawed frog CNN3, NP_989257.1; Western terrestrial garter snake CNN2, XP_032064388.1; Western terrestrial garter snake CNN3, XP_032074010.1; Zebrafish CNN1, XP_701038.5; Zebrafish CNN2, NP_ 998514.1; Zebrafish CNN3, NP_956047.1.

    Article Snippet: The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method.

    Techniques: Generated, Software, Sequencing, Derivative Assay, Western Blot

    FIGURE 2 Phylogenetic tree of vertebrate transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. TAGLN, TAGLN2, and TAGLN3 isoforms are marked in purple, red and green, respectively. The NCBI database accession numbers of the sequences analyzed are: African clawed frog TAGLN, NP_001083600.1; African clawed frog TAGLN2 NP_001080783.1; African turquoise killifish TAGLN3, KAF7213900.1; Black-legged kittiwake TAGLN, XP_054078918.1; Black-legged kittiwake TAGLN2, XP_054038817.1; Black-legged kittiwake TAGLN3, XP_054071416.1; Cape cliff lizard TAGLN, XP_053124973.1; Cape cliff lizard TAGLN2, XP_053133737.1; Cape cliff lizard TAGLN3, XP_053167629.1; Chicken TAGLN, AAA48782.1; Chicken TAGLN2, XP_024999416.1; Chicken TAGLN3, XP_040518015.1; Diamondback rattlesnake TAGLN, JAI10881.1; European plaice TAGLN, XP_053298355.1; European seabass TAGLN, XP_051257332.1; Green swordtail TAGLN3, KAF5894368.1; Human TAGLN, NP_001001522.1; Human TAGLN2, KAI4083433.1; Human TAGLN3, KAI2530827.1; Mexican tetra TAGLN2, XP_049332440.1; Mexican tetra TAGLN3, KAG9283353.1; Mouse TAGLN, CAA92941.1; Mouse TAGLN2, NP_848713.1; Mouse TAGLN3, AAH55338.1; Orange spotted grouper TAGLN, ABW04145.1; Tiger rattlesnake TAGLN, 2, XP_039222054.1; Tiger rattlesnake TAGLN3, XP_039220015.1; Tiny caecilian TAGLN, XP_030076731.1; Tiny caecilian TAGLN2, XP_030043052.1; Tiny caecilian TAGLN3, XP_030060064.1; Two-lined caecilian TAGLN, XP_029429188.1; Two-lined caecilian TAGLN2, XP_XP_ 029436331.1; Two-lined caecilian TAGLN3, XP_029434652.1; Western clawed frog TAGLN, NP_001025579.1; Western clawed frog TAGLN2, NP_ 989354.1; Yellow drum fish TAGLN, KAG8008337.1.

    Journal: Frontiers in cell and developmental biology

    Article Title: Evolution and function of calponin and transgelin.

    doi: 10.3389/fcell.2023.1206147

    Figure Lengend Snippet: FIGURE 2 Phylogenetic tree of vertebrate transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. TAGLN, TAGLN2, and TAGLN3 isoforms are marked in purple, red and green, respectively. The NCBI database accession numbers of the sequences analyzed are: African clawed frog TAGLN, NP_001083600.1; African clawed frog TAGLN2 NP_001080783.1; African turquoise killifish TAGLN3, KAF7213900.1; Black-legged kittiwake TAGLN, XP_054078918.1; Black-legged kittiwake TAGLN2, XP_054038817.1; Black-legged kittiwake TAGLN3, XP_054071416.1; Cape cliff lizard TAGLN, XP_053124973.1; Cape cliff lizard TAGLN2, XP_053133737.1; Cape cliff lizard TAGLN3, XP_053167629.1; Chicken TAGLN, AAA48782.1; Chicken TAGLN2, XP_024999416.1; Chicken TAGLN3, XP_040518015.1; Diamondback rattlesnake TAGLN, JAI10881.1; European plaice TAGLN, XP_053298355.1; European seabass TAGLN, XP_051257332.1; Green swordtail TAGLN3, KAF5894368.1; Human TAGLN, NP_001001522.1; Human TAGLN2, KAI4083433.1; Human TAGLN3, KAI2530827.1; Mexican tetra TAGLN2, XP_049332440.1; Mexican tetra TAGLN3, KAG9283353.1; Mouse TAGLN, CAA92941.1; Mouse TAGLN2, NP_848713.1; Mouse TAGLN3, AAH55338.1; Orange spotted grouper TAGLN, ABW04145.1; Tiger rattlesnake TAGLN, 2, XP_039222054.1; Tiger rattlesnake TAGLN3, XP_039220015.1; Tiny caecilian TAGLN, XP_030076731.1; Tiny caecilian TAGLN2, XP_030043052.1; Tiny caecilian TAGLN3, XP_030060064.1; Two-lined caecilian TAGLN, XP_029429188.1; Two-lined caecilian TAGLN2, XP_XP_ 029436331.1; Two-lined caecilian TAGLN3, XP_029434652.1; Western clawed frog TAGLN, NP_001025579.1; Western clawed frog TAGLN2, NP_ 989354.1; Yellow drum fish TAGLN, KAG8008337.1.

    Article Snippet: The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method.

    Techniques: Generated, Western Blot

    FIGURE 3 Phylogenetic tree of invertebrate calponin isoforms. The phylogenetic tree of invertebrate calponin was generated by aligning the amino acid sequences of annotated calponin isoforms in the representative invertebrate species with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. Calponin isoforms 1, 2, and 3 are indicated in purple, red and green fonts, respectively. The NCBI database accession numbers of the sequences analyzed are: Bark spider CNN2, GIY95920.1; Bat sea star CNN1, XP_038068192.1; Bat sea star CNN3, XP_038068193.1; Belted springtail CNN1, ODN05277.1; Bird tapeworm CNN3, JAP59453.1; Blood fluke CNN3, XP_012795279.2; Dog round worm CNN2, KHN73181.1; Dog tapeworm CNN1, KAH9282893.1; Dog tapeworm CNN3, EUB56891.1; East Asian octopus CNN1, XP_029648512.1; Fungivorous round worm CNN2, KAH7720906.1; Garden spider CNN3, GBN55272.1; Gray mussel CNN2, APB61452.1; Hood coral CNN1, PFX34187.1; Hood coral CNN3, PFX34186.1; Human whip worm CNN1, CDW60276.1; Intoshia linei worm CNN1, OAF71457.1; Japanese scallop CNN3, OWF41392.1; Oriental liver fluke CNN3, GAA43144.2; Pale anemone CNN1,KXJ22199.1; Taxon placozoa CNN1, RDD43626.1; Water flea CNN3, JAM94970.1; White springtail CNN1, OXA37663.1; Satsuma tube worm CNN3, KAI0207068.1; Salmon louse CNN3, ACO12641.1; Sea louse CNN3, ACO14747.1; Sea spider CNN3, KAG1690965.1; Spider mite CNN3, XP_015791637.1.

    Journal: Frontiers in cell and developmental biology

    Article Title: Evolution and function of calponin and transgelin.

    doi: 10.3389/fcell.2023.1206147

    Figure Lengend Snippet: FIGURE 3 Phylogenetic tree of invertebrate calponin isoforms. The phylogenetic tree of invertebrate calponin was generated by aligning the amino acid sequences of annotated calponin isoforms in the representative invertebrate species with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method. The degrees of evolutionary divergence are indicated by the lengths of lineage lines. Calponin isoforms 1, 2, and 3 are indicated in purple, red and green fonts, respectively. The NCBI database accession numbers of the sequences analyzed are: Bark spider CNN2, GIY95920.1; Bat sea star CNN1, XP_038068192.1; Bat sea star CNN3, XP_038068193.1; Belted springtail CNN1, ODN05277.1; Bird tapeworm CNN3, JAP59453.1; Blood fluke CNN3, XP_012795279.2; Dog round worm CNN2, KHN73181.1; Dog tapeworm CNN1, KAH9282893.1; Dog tapeworm CNN3, EUB56891.1; East Asian octopus CNN1, XP_029648512.1; Fungivorous round worm CNN2, KAH7720906.1; Garden spider CNN3, GBN55272.1; Gray mussel CNN2, APB61452.1; Hood coral CNN1, PFX34187.1; Hood coral CNN3, PFX34186.1; Human whip worm CNN1, CDW60276.1; Intoshia linei worm CNN1, OAF71457.1; Japanese scallop CNN3, OWF41392.1; Oriental liver fluke CNN3, GAA43144.2; Pale anemone CNN1,KXJ22199.1; Taxon placozoa CNN1, RDD43626.1; Water flea CNN3, JAM94970.1; White springtail CNN1, OXA37663.1; Satsuma tube worm CNN3, KAI0207068.1; Salmon louse CNN3, ACO12641.1; Sea louse CNN3, ACO14747.1; Sea spider CNN3, KAG1690965.1; Spider mite CNN3, XP_015791637.1.

    Article Snippet: The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method.

    Techniques: Generated

    FIGURE 4 Phylogenetic tree of invertebrate animal and fungal transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of transgelin isoforms of representative invertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are listed in purple, red and green fonts, respectively. Fungal transgelins are highlighted in brown font. The NCBI database accession numbers of the sequences are: California two spot octopus TAGLN3, XP_014777580.1; Caenorhabditis elegans TAGLN, NP_ 493713.2; Common house spider TAGLN, LAA01862.1; Common starfish TAGLN, QAA95982.1; Darwini’s bark spider TAGLN3, GIY80101.1; Dog tapeworm TAGLN, KAH9282095.1; Oriental liver fluke TAGLN, KAG5444608.1; Freshwater snail TAGLN3, KAH9519225.1; Hood coral TAGLN2, PFX34185.1; Human scabies TAGLN2, KAI7688587.1; Japanese scallop TAGLN3, OWF50751.1; Mediterranean mussel TAGLN, VDI10566.1; Oriental lung fluke TAGLN, KAA3679206.1; Pharaoh cuttlefish TAGLN, CAE1295289.1; Pork worm TAGLN2, KRY33709.1; Satsuma tube worm TAGLN2, KAI0231824.1; Sea slug TAGLN, GFO13790.1; Silkworm moth TAGLN, ABF51271.1; Speckled wood butterfly TAGLN, JAA92459.1; Strawberry crimp disease nematode TAGLN, KAI6193121.1; Water flea TAGLN3, JAJ10604.1; Aspergillus luchuensis TAGLN, GAT26957.1; Budding yeast TAGLN, KAF4004496.1; Candida parapsilosis TAGLN, KAI5906036.1; Fission yeast TAGLN, O14185.1.

    Journal: Frontiers in cell and developmental biology

    Article Title: Evolution and function of calponin and transgelin.

    doi: 10.3389/fcell.2023.1206147

    Figure Lengend Snippet: FIGURE 4 Phylogenetic tree of invertebrate animal and fungal transgelin isoforms. The phylogenetic tree was generated by aligning amino acid sequences of transgelin isoforms of representative invertebrate species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are listed in purple, red and green fonts, respectively. Fungal transgelins are highlighted in brown font. The NCBI database accession numbers of the sequences are: California two spot octopus TAGLN3, XP_014777580.1; Caenorhabditis elegans TAGLN, NP_ 493713.2; Common house spider TAGLN, LAA01862.1; Common starfish TAGLN, QAA95982.1; Darwini’s bark spider TAGLN3, GIY80101.1; Dog tapeworm TAGLN, KAH9282095.1; Oriental liver fluke TAGLN, KAG5444608.1; Freshwater snail TAGLN3, KAH9519225.1; Hood coral TAGLN2, PFX34185.1; Human scabies TAGLN2, KAI7688587.1; Japanese scallop TAGLN3, OWF50751.1; Mediterranean mussel TAGLN, VDI10566.1; Oriental lung fluke TAGLN, KAA3679206.1; Pharaoh cuttlefish TAGLN, CAE1295289.1; Pork worm TAGLN2, KRY33709.1; Satsuma tube worm TAGLN2, KAI0231824.1; Sea slug TAGLN, GFO13790.1; Silkworm moth TAGLN, ABF51271.1; Speckled wood butterfly TAGLN, JAA92459.1; Strawberry crimp disease nematode TAGLN, KAI6193121.1; Water flea TAGLN3, JAJ10604.1; Aspergillus luchuensis TAGLN, GAT26957.1; Budding yeast TAGLN, KAF4004496.1; Candida parapsilosis TAGLN, KAI5906036.1; Fission yeast TAGLN, O14185.1.

    Article Snippet: The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method.

    Techniques: Generated, Software

    FIGURE 8 Phylogeny of the calponin-transgelin superfamily genes in vertebrates. The combined phylogenetic tree of vertebrate calponin and transgelin isoforms was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are marked in purple, red and green fonts, respectively. The vertebrate calponin isoforms 1, 2, and 3 are clustered in the blue box. The phylogenetic lineages demonstrate that calponin and transgelin diverged early during vertebrate evolution and transgelin-1 emerged before transgelin-2, transgelin-3 and the three calponin isoforms. The NCBI database accession numbers of the sequences are listed in the legends of Figures 1, 2.

    Journal: Frontiers in cell and developmental biology

    Article Title: Evolution and function of calponin and transgelin.

    doi: 10.3389/fcell.2023.1206147

    Figure Lengend Snippet: FIGURE 8 Phylogeny of the calponin-transgelin superfamily genes in vertebrates. The combined phylogenetic tree of vertebrate calponin and transgelin isoforms was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Transgelin isoforms 1, 2, and 3 are marked in purple, red and green fonts, respectively. The vertebrate calponin isoforms 1, 2, and 3 are clustered in the blue box. The phylogenetic lineages demonstrate that calponin and transgelin diverged early during vertebrate evolution and transgelin-1 emerged before transgelin-2, transgelin-3 and the three calponin isoforms. The NCBI database accession numbers of the sequences are listed in the legends of Figures 1, 2.

    Article Snippet: The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method.

    Techniques: Generated, Software

    FIGURE 9 Overall phylogeny of calponin-transgelin genes in vertebrate and invertebrate animals. The combined phylogenetic tree of vertebrate and invertebrate calponin and transgelin was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Vertebrate calponin isoforms are in blue font, invertebrate calponins are in black font, vertebrate transgelins are in green font and invertebrate transgelins are in red font. The NCBI database accession numbers of the sequences are in the legends of Figures 1–4. The evolutionary lineages show that while calponin and transgelin have evolved into distinct and conserved isoforms in vertebrates, the invertebrate homologs are significantly less differentiated.

    Journal: Frontiers in cell and developmental biology

    Article Title: Evolution and function of calponin and transgelin.

    doi: 10.3389/fcell.2023.1206147

    Figure Lengend Snippet: FIGURE 9 Overall phylogeny of calponin-transgelin genes in vertebrate and invertebrate animals. The combined phylogenetic tree of vertebrate and invertebrate calponin and transgelin was generated by aligning amino acid sequences of representative species with the DNASTAR MegAlign computer software (Lasergene, lnc, Madison, WI) using Clustal W method. Vertebrate calponin isoforms are in blue font, invertebrate calponins are in black font, vertebrate transgelins are in green font and invertebrate transgelins are in red font. The NCBI database accession numbers of the sequences are in the legends of Figures 1–4. The evolutionary lineages show that while calponin and transgelin have evolved into distinct and conserved isoforms in vertebrates, the invertebrate homologs are significantly less differentiated.

    Article Snippet: The phylogenetic tree was generated by aligning amino acid sequences of the three transgelin isoforms in representative vertebrate classes including fish, amphibian, reptile, avian, and mammal with the MegAlign computer program (Lasergene; DNASTAR, lnc, Madison, WI) using Clustal W method.

    Techniques: Generated, Software